Coding

Part:BBa_K4010004:Design

Designed by: iGEM_McMaster   Group: iGEM21_McMaster   (2021-10-10)


TorA-Linked Colicin E1 and Immunity Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1833
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 133
    Illegal SapI site found at 439


Design Notes

The space between E1 and the linker is an overlapping section required for the Gibson assembly of two strands with a primer.


Source

Igem parts registry

References